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Tg(CAG-Ub*G76V/GFP)2Dant
Transgene Detail
Nomenclature
Symbol: Tg(CAG-Ub*G76V/GFP)2Dant
Name: transgene insertion 2, Nico Dantuma
MGI ID: MGI:3762796
Synonyms: Tg(ACTB-Ub*G76V/GFP)2Dant, Tg(CAG-Ub*G76V/GFP)2Dant, UbG76V-GFP/2, UbG76V-GFP/2, UPS reporter
Transgene: Tg(CAG-Ub*G76V/GFP)2Dant  Location: unknown  
Transgene
origin
Strain of Origin:  (CBA x C57BL/6)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V). This transgene was microinjected into the pronucleus of fertilized (CBA x C57BL/6)F1 oocytes. Although transcripts of the UbG76V-GFP fusion gene are detected in all tissues examined, the G76V substitution prevents removal of the ubiquitin moiety leading to efficient ubiquitination and proteasomal degradation of the fusion protein (and thus no fluorescence) in these tissues. Line 2 with low expression was maintained by backcross to C57BL/6N mice. (J:127085, J:127087)
Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:127085 Lindsten K, et al., A transgenic mouse model of the ubiquitin/proteasome system. Nat Biotechnol. 2003 Aug;21(8):897-902
All:  12 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory