Tg(tetO-Vamp2)1Kdmc
Transgene Detail

Summary

• Symbol: Tg(tetO-Vamp2)1Kdmc
• Name: transgene insertion 1, Ken D McCarthy
• MGI ID: MGI:3762765
• Synonyms: dn-SNARE, dominant-negative SNARE, tetO.SNARE
• Transgene: Tg(tetO-Vamp2)1Kdmc Location: unknown
• Alliance: Tg(tetO-Vamp2)1Kdmc page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgene contains the tet operator, tetO, regulating expression of a dominant negative N-ethylmaleimide-sensitive factor attachment protein receptor, or SNARE (dn-SNARE), that blocks the release of neurotransmitters. dn-SNARE is the cytosolic part of the SNARE domain (amino acids 1-96) of vesicle-associated membrane protein 2 (VAMP2; formerly synaptobrevin II). The enhanced green fluorescent protein (EGFP) and E. coli beta-galactosidase (lacZ) reporter genes are also expressed under the control of tetO. The three constructs were co-injected. (J:102245)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:102245 Pascual O, et al., Astrocytic purinergic signaling coordinates synaptic networks. Science. 2005 Oct 7;310(5745):113-6
• All: 23 reference(s)