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Tg(Thy1-Brainbow1.0)LLich
Transgene Detail
Nomenclature
Symbol: Tg(Thy1-Brainbow1.0)LLich
Name: transgene insertion L, Jeff W Lichtman
MGI ID: MGI:3761287
Synonyms: Brainbow1.0L, Tg(Thy1-dTomato,-mCerulean,-mYFP)L10Lich, Thy1-Brainbow-1.0 line L
Transgene: Tg(Thy1-Brainbow1.0)LLich  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6 x CBA
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), cyan (CFP), or enhanced yellow (EYFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5' to 3') a Lox2272 site, LoxP site, dTomato RFP (with polyA sequence), Lox2272 site, mCerulean CFP (with polyA sequence), LoxP site, monomeric EYFP (mYFPA206K; with 5' FRT site and polyA sequence). The dTomato RFP is an optimized DsRed fluorescent protein variant with a combination of amino acid substitutions (V22M/Q66M/V105L/F124M) designed to improve spectral properties. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. (J:125961)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
References
Original:  J:125961 Livet J, et al., Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature. 2007 Nov 1;450(7166):56-62
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/13/2021
MGI 6.16
The Jackson Laboratory