Igh^tm1.2Rsky
Targeted Allele Detail

Summary

• Symbol: Igh^tm1.2Rsky
• Name: immunoglobulin heavy chain complex; targeted mutation 1.2, Klaus Rajewsky
• MGI ID: MGI:3760662
• Synonyms: IgH^gamma1mu
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125719
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Intergenic deletion, Inversion
• Mutation details: These mice are derived from chimeric mice produced during creation of the Igh^tm1.1Rsky mouse strain. Upon crossing the chimeras with Cre expressing transgenic mice, the CH region of the Igh locus is deleted and an inversion of the targeted construct also occurs. This places the mutant Ighg1 into the right orientation to express functional protein. B cells from these mice were found to exclusively express IgG1 antibodies in both a soluble and membrane bound form. (J:125719)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:125719 Waisman A, et al., IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta. J Exp Med. 2007 Apr 16;204(4):747-58
• All: 2 reference(s)