About   Help   FAQ
Ightm1.1Rsky
Targeted Allele Detail
Summary
Symbol: Ightm1.1Rsky
Name: immunoglobulin heavy chain complex; targeted mutation 1.1, Klaus Rajewsky
MGI ID: MGI:3760655
Synonyms: IgHmugamma1
Gene: Igh  Location: Chr12:113222388-115973574 bp, - strand  Genetic Position: Chr12, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:125719
Parent Cell Line:  BALB/c-I (ES Cell)
Strain of Origin:  BALB/cJ
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Deletion, Insertion
  Ightm1.1Rsky involves 1 genes/genome features (Igh) View all
 
Mutation detailsA DNA segment containing a IgH-6 gene (heavy chain of IgM) and an inverted Ighg1 (heavy constant gamma 1 chain) was inserted immediately downstream of the constant heavy chain gene region (CH). To restrict expression of soluble isoforms, the relevant polyA sites were mutated (Ighg1) or eliminated (Igh-6). LoxP sites in opposite orientations were placed around the genes and a neomycin resistance cassette was placed upstream of the entire construct. This construct was used in targeting of stem cells from Igh-6tm1Fbb mice in which the Igh-6 locus is replaced with a loxP site. Chimeric mice were crossed with transgenic mice expressing Cre recombinase, eliminating the entire CH region and the neo resistance cassette while leaving the mutant IgH-6 gene intact and in the right orientation to express. B cells from these mice were confirmed to exclusively express membrane bound IgM antibody. (J:125719)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Igh Mutation:  43 strains or lines available
References
Original:  J:125719 Waisman A, et al., IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta. J Exp Med. 2007 Apr 16;204(4):747-58
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory