Rapgef6^tm1Tkat
Targeted Allele Detail

Summary

• Symbol: Rapgef6^tm1Tkat
• Name: Rap guanine nucleotide exchange factor (GEF) 6; targeted mutation 1, Tohru Kataoka
• MGI ID: MGI:3760542
• Synonyms: RA-GEF-2^flox
• Gene: Rapgef6 Location: Chr11:54413673-54590111 bp, + strand Genetic Position: Chr11, 32.13 cM
• Alliance: Rapgef6^tm1Tkat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126164
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 21 and a floxed neo cassette downstream of exon 21 to create a floxed allele in EB3 ES cells. Insertion was confirmed by Southern blot analysis. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. (J:126164)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rapgef6 Mutation: 107 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:126164 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:126164 Yoshikawa Y, et al., The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes. Mol Biol Cell. 2007 Aug;18(8):2949-59
• All: 2 reference(s)