Cdk8^{GT(RRS314)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Cdk8^{GT(RRS314)Byg}
• Name: cyclin dependent kinase 8; gane trap RRS314, BayGenomics
• MGI ID: MGI:3760076
• Synonyms: Cdk8^-, Cdk8^{GT(pGT0Lxf)/Byg}
• Gene: Cdk8 Location: Chr5:146168040-146239684 bp, + strand Genetic Position: Chr5, 85.19 cM
• Alliance: Cdk8^{GT(RRS314)Byg} page

Mutation origin

• Mutant Cell Line: RRS314
• Germline Transmission: Earliest citation of germline transmission: J:125327
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT0Lxf was inserted into intron 4 and is predicted to generate a fusion protein of 163 kDa with 152 N-terminal amino acids from Cdk8 fused to 1323 amino acids of the beta-geo selection marker. The authors state that this allele is a functional null and the protein product has no kinase activity. LacZ expression was confirmed by a beta-galactosidase assay. (J:125327)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRS314	CG784396 ; (MGI Seq Detail)	5' RACE	Chr5:146208413-146208543 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  23 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk8 Mutation: 49 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:125327 Westerling T, et al., Cdk8 is essential for preimplantation mouse development. Mol Cell Biol. 2007 Sep;27(17):6177-82
• All: 2 reference(s)