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Transgene Detail
Symbol: Tg(CAG-EGFP/Map1lc3b)53Nmz
Name: transgene insertion 53, Noboru Mizushima
MGI ID: MGI:3759813
Synonyms: CAG-GFP-LC3, GFP-LC3, GFP-LC3#53, GFP-LC3B, LC3-GFP, Tg(ACTB-EGFP/Map1lc3b)53Nmz, Tg(CAG-EGFP/M1lc3b)53Nmz, Tg(CAG-EGFP/Map1lc3b)53Nmz
Transgene: Tg(CAG-EGFP/Map1lc3b)53Nmz  Location: unknown  Genetic Position: Chr2, Syntenic
Strain of Origin:  C57BL/6NCrlj x (C57BL/6 x DBA/2)F1
Transgene Type:    Transgenic (Inserted expressed sequence, Reporter)
Mutation:    Insertion
Tg(CAG-EGFP/Map1lc3b)53Nmz expresses 1 gene
Mutation detailsThe transgene comprises a fusion cDNA encoding enhanced green fluorescent protein joined at its C-terminus to rat microtubule-associated protein 1 light chain 3 beta under control of the "CAG promoter" -- which consists of the cytomegalovirus immediate-early enhancer (CMV-EI) followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene -- and followed by the SV40 late polyadenylation signal. Expression of the transgene is observed during prenatal stages and in nearly all tissues of the adult mouse. Its level is similar to that of endogenous MAP1LC3B in brain, but significantly higher in other tissues. In liver of fed mice, it occurs diffusely throughout the cytoplasm; upon starvation, it becomes localized to isolation membranes and phagosomes. Cytoplamsic aggregations of this fusion protein indicate autophagic activity. The transgene insertion site is located in the distal region of chromosome 2, 106 bp upstream of the Gm14314 locus. (J:114076, J:268663, J:276483)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Of 8 transgenic lines, only #53 is named or described. (J:114076)
Original:  J:114076 Mizushima N, et al., In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell. 2004 Mar;15(3):1101-11
All:  189 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
The Jackson Laboratory