Cyp1a2^tm3Dwn
Targeted Allele Detail

Summary

• Symbol: Cyp1a2^tm3Dwn
• Name: cytochrome P450, family 1, subfamily a, polypeptide 2; targeted mutation 3, Daniel W Nebert
• MGI ID: MGI:3722076
• Synonyms: Cyp1a2(t)
• Gene: Cyp1a2 Location: Chr9:57584220-57590938 bp, - strand Genetic Position: Chr9, 31.3 cM
• Alliance: Cyp1a2^tm3Dwn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:122409
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting construct with a long arm for recombination encompassing 4429 bp of Cyp1a2 genomic sequence plus 48 bp of 3'-flanking sequence was followed by a floxed PGK-NEO gene and then by 1452 bp of 3'-flanking sequence for the short arm. An HSK-TK negative-selection cassette was placed outside the 3' Cyp1a2 homology arm. This construct was electroporated into 129S6/SvEvTac ES cells. In recombined ES cells, an allele containing a floxed PGK-NEO gene, placed 350 bp 3' of the stop codon and a loxP site located 48 bp 3' beyond the end of exon 7 was generated. (J:122409)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp1a2 Mutation: 35 strains or lines available

References

• Original: J:122409 Dragin N, et al., Generation of 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-) mouse line. Biochem Biophys Res Commun. 2007 Aug 3;359(3):635-42
• All: 1 reference(s)