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Cd28tm1Shr
Targeted Allele Detail
Nomenclature
Symbol: Cd28tm1Shr
Name: CD28 antigen; targeted mutation 1, Arlene H Sharpe
MGI ID: MGI:3721239
Synonyms: Cd28-
Gene: Cd28  Location: Chr1:60785547-60812521 bp, + strand  Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:68642
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA PGK-hygro cassette replaced a 3 kb region encompassing portions of exon 2 and exon 3. The deleted region encoded parts of a IgV-like domain and a transmembrane domain. Fluorescence-activated cell sorting (FACS) analysis showed an absence of protein on naive CD4 T cells isolated from the lymph nodes of homozygous mutant mice. Targeting was performed on an embryonic stem cell clone that was heterozygote for a targeted mutation of Ctla4tm1Shr. (J:68642)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Cd28 Mutation:  38 strains or lines available
References
Original:  J:68642 Mandelbrot DA, et al., B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4. J Clin Invest. 2001 Apr;107(7):881-7
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory