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Tg(Ckm-APPSw)A6Lfa
Transgene Detail
Nomenclature
Symbol: Tg(Ckm-APPSw)A6Lfa
Name: transgene insertion A6, Frank M LaFerla
MGI ID: MGI:3720796
Synonyms: A6 line, Tg(Ckm-APP*K670N*M671L)A6Lfa
Transgene: Tg(Ckm-APPSw)A6Lfa  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6 x SJL
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Ckm-APPSw)A6Lfa expresses 1 gene
 
Mutation detailsHuman APP cDNA harboring the Swedish double mutation (K670N,M671L) was inserted downstream of the 1.3 kb 5' flanking sequence of the mouse muscle creatine kinase Ckm, upstream of the SV40 polyadenylation signal. The 3.8 kb construct was microinjected into pronuclei of single-cell C57Bl/6 x SJL embryos. Northern blot showed relatively high expression of the transgene in skeletal muscle of line A6 mice, approximately 12 times higher than in Tg(Ckm-APP*K670N*M671L)A2Lfa mice. Western blot showed that transgenic protein expression is ~9 times higher than in mice from line A2. (J:76338)
Phenotypes
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Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:76338 Sugarman MC, et al., Inclusion body myositis-like phenotype induced by transgenic overexpression of beta APP in skeletal muscle. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6334-9
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
04/21/2015
MGI 5.21
The Jackson Laboratory