Esrrbtm1.1Nat
Targeted Allele Detail
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Symbol: |
Esrrbtm1.1Nat |
Name: |
estrogen related receptor, beta; targeted mutation 1.1, Jeremy Nathans |
MGI ID: |
MGI:3720481 |
Synonyms: |
CKOflp, Nr3b2CKO |
Gene: |
Esrrb Location: Chr12:86407891-86568402 bp, + strand Genetic Position: Chr12, 40.49 cM
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Alliance: |
Esrrbtm1.1Nat page
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Germline Transmission: |
Earliest citation of germline transmission:
J:124941
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutation: |
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Insertion
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Mutation details: A targeting vector was designed to place a loxp site 184 bp upstream, as well as a loxP site 123 bp downstream of exon 2 of the targeted gene. An FRT-flanked PGK-neo cassette was also placed just downstream of the 3' loxP site. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 (to generate Nr3b2CKO+neo mice). The neomycin selection cassette was excised by crossing to mice constitutively expressing the Flp recombinase (unspecified genetic background, see Stock No. 003800), generating this Nr3b2CKO allele with a single FRT site remaining.
(J:124941)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:124941 Chen J, et al., Estrogen-Related Receptor beta/NR3B2 Controls Epithelial Cell Fate and Endolymph Production by the Stria Vascularis. Dev Cell. 2007 Sep;13(3):325-37 |
All: |
6 reference(s) |
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