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Transgene Detail
Symbol: Tg(Thy1-PSEN1*A246E)2Vln
Name: transgene insertion 2, Fred Van Leuven
MGI ID: MGI:3717624
Synonyms: Ps1 A246E, Ps1/A246E, PS1[A246E], PS1mut
Transgene: Tg(Thy1-PSEN1*A246E)2Vln  Location: unknown  
Strain of Origin:  FVB/N
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Thy1-PSEN1*A246E)2Vln expresses 1 gene
Mutation detailscDNA encoding the human PSEN1 sequence containing the A246E mutation was ligated into the mouse Thy1 gene. In the final construct, coding sequences and introns 1 and 2 of the Thy1 gene are replaced by the mutant PSEN1 sequence, with the 5' neuron-specific control sequences of Thy1 intact. Linearized minigenes are microinjected into prenuclear FVB/N embryos. Mutant PSEN1 mRNA and protein are detected in total brain extracts by Northern and Western blotting, respectively. (J:64209, J:100968)
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Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
J:100968 discusses generation of trangenic PSEN1[A246E] mice, referencing J:64209 as one paper on the method. Since the transgenic mice are apparently identical, it was assumed that new transgenic mice were not created, but mice are actually the ones referenced in J:64209. -rbabiuk 8/9/2007
Original:  J:64209 Dewachter I, et al., Aging increased amyloid peptide and caused amyloid plaques in brain of old APP/V717I transgenic mice by a different mechanism than mutant presenilin1. J Neurosci. 2000 Sep 1;20(17):6452-8
All:  11 reference(s)

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MGI 6.04
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