Kif11^Gt(XE579)Byg
Gene trapped Allele Detail

Summary

• Symbol: Kif11^Gt(XE579)Byg
• Name: kinesin family member 11; gene trap XE579, BayGenomics
• MGI ID: MGI:3711986
• Synonyms: Eg5^-
• Gene: Kif11 Location: Chr19:37364851-37410307 bp, + strand Genetic Position: Chr19, 32.27 cM
• Alliance: Kif11^Gt(XE579)Byg page

Mutation origin

• Mutant Cell Line: XE579
• Germline Transmission: Earliest citation of germline transmission: J:121799
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector containing a beta-galactosidase/neomycin gene was inserted within intron 18. RT-PCR confirmed that mutants contained a truncated mRNA resulting from the splicing of exon 18 to the beta-geo cassette using the cryptic splice site from the gene trap vector. The resulting protein is predicted to be inactive as it lacks the 129 amino acids of the tail domain. (J:121799)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE579	CC183381 ; (MGI Seq Detail)	5' RACE	Chr19:37399872-37401787 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  42 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif11 Mutation: 59 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:121799 Castillo A, et al., The kinesin related motor protein, Eg5, is essential for maintenance of pre-implantation embryogenesis. Biochem Biophys Res Commun. 2007 Jun 8;357(3):694-9
• All: 3 reference(s)