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Targeted Allele Detail
Symbol: Sphk2tm1.1Cgh
Name: sphingosine kinase 2; targeted mutation 1.1, Shaun R Coughlin
MGI ID: MGI:3708000
Gene: Sphk2  Location: Chr7:45709467-45718002 bp, - strand  Genetic Position: Chr7, 29.45 cM
Impaired lymphocyte egress in Lyve1tm1.1(EGFP/cre)Cys/Lyve1+ Sphk1tm2Cgh/Sphk1tm2Cgh Sphk2tm1.1Cgh/Sphk2tm1.1Cgh mice

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:120783
Parent Cell Line:  RF8 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsAn FRT-flanked neo cassette with a single 3' loxP site was inserted between exons 1 and 2 and an additional loxP site was inserted downstream of exon 5. The neo cassette was subsequently removed through flp mediated recombination. In vivo Cre-mediated recombination removed exons 2 through 5 creating a null allele. The loss of transcript was confirmed by RT-PCR. (J:120783)
Generation of the Lyve1tm1.1(EGFP/cre)Cys allele
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sphk2 Mutation:  24 strains or lines available
Original:  J:120783 Pappu R, et al., Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate. Science. 2007 Apr 13;316(5822):295-8
All:  8 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.10
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