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Targeted Allele Detail
Symbol: Sphk2tm1.1Cgh
Name: sphingosine kinase 2; targeted mutation 1.1, Shaun R Coughlin
MGI ID: MGI:3708000
Gene: Sphk2  Location: Chr7:45709467-45718002 bp, - strand  Genetic Position: Chr7, 29.45 cM
Impaired lymphocyte egress in Lyve1tm1.1(EGFP/cre)Cys/Lyve1+ Sphk1tm2Cgh/Sphk1tm2Cgh Sphk2tm1.1Cgh/Sphk2tm1.1Cgh mice

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:120783
Parent Cell Line:  RF8 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsAn FRT-flanked neo cassette with a single 3' loxP site was inserted between exons 1 and 2 and an additional loxP site was inserted downstream of exon 5. The neo cassette was subsequently removed through flp mediated recombination. In vivo Cre-mediated recombination removed exons 2 through 5 creating a null allele. The loss of transcript was confirmed by RT-PCR. (J:120783)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sphk2 Mutation:  24 strains or lines available
Original:  J:120783 Pappu R, et al., Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate. Science. 2007 Apr 13;316(5822):295-8
All:  8 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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