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Foxp3tm3Tch
Targeted Allele Detail
Nomenclature
Symbol: Foxp3tm3Tch
Name: forkhead box P3; targeted mutation 3, Talal A Chatila
MGI ID: MGI:3707723
Synonyms: Foxp3deltaEGFP, Foxp3-GFP
Gene: Foxp3  Location: ChrX:7579676-7595243 bp, + strand  Genetic Position: ChrX, 3.41 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:120636
Parent Cell Line:  SCC10 (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsEGFP cDNA followed by a stop codon was inserted in-frame after codon 396 in exon 11 of Foxp3 and a loxP-flanked PGK-Neo was inserted in exon 9 in the same orientation of Foxp3. A diphtheria toxin cassette for negative selection was included in the targeting construct. Correctly targeted chimeric males were crossed with Cre-deleter mice on the BALB/c background to remove the PGK-cassette. Mice express a mutant Foxp3/EGFP protein with the 33 amino acid C-terminal peptide residues including DNA- and transcription factor-binding and a nuclear localization sequence replaced by EGFP under control of the endogenous promoter (J:120636)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Foxp3 Mutation:  45 strains or lines available
References
Original:  J:120636 Lin W, et al., Regulatory T cell development in the absence of functional Foxp3. Nat Immunol. 2007 Apr;8(4):359-68
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory