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Hprt1tm2(Penk1-lacZ)Saub
Targeted Allele Detail
Summary
Symbol: Hprt1tm2(Penk1-lacZ)Saub
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Brian Sauer
MGI ID: MGI:3702336
Synonyms: ENK5.0
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm2(Penk1-lacZ)Saub page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:81778
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA neo cassette with a 3' loxP site followed by an alkaline phosphatase gene with a 3' loxP site was inserted into exon 3. Transient cre expression was used to knockin transgene composed of 5.0kp of promoter region and 600bp of 5'UTR and intron 1 of Penk1 fused to a lacZ cassette along with a portion of the Penk1 3'UTR between the alkaline phosphatase gene and the second loxP site. Expression of the insertion was confirmed by RT-PCR of the brain, heart and liver all of which expressed lacZ mRNA. (J:81778)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1272 strains or lines available
References
Original:  J:81778 Le Y, et al., Far-upstream elements are dispensable for tissue-specific proenkephalin expression using a Cre-mediated knock-in strategy. J Neurochem. 2003 Feb;84(4):689-97
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory