Hprt1^{tm1(Penk1-lacZ)Saub}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Penk1-lacZ)Saub}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Brian Sauer
• MGI ID: MGI:3702333
• Synonyms: ENK1.4
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Penk1-lacZ)Saub} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81778
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A neo cassette with a 3' loxP site followed by an alkaline phosphatase gene with a 3' loxP site was inserted into exon 3. Transient cre expression was used to knockin a transgene composed of 1.4kb of the promoter of Penk1, 600bp of the 5' UTR and first intron of Penk1 fused to a LacZ cassette and the 3'UTR of Penk1 between the alkaline phosphatase gene and the second loxP site. RT-PCR confirmed transgene expression of LacZ mRNA in brain, heart and liver tissue. (J:81778)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

References

• Original: J:81778 Le Y, et al., Far-upstream elements are dispensable for tissue-specific proenkephalin expression using a Cre-mediated knock-in strategy. J Neurochem. 2003 Feb;84(4):689-97
• All: 1 reference(s)