About   Help   FAQ
Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm1(rtTA*M2)Jae
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch
MGI ID: MGI:3702294
Synonyms: Gt(ROSA)26Sortm1(M2rtTA)Jae, M2-rtTA, R26-M2rtTA, R26-rtTA, Rosa26-M2rtTA, Rosa26-rtRA-nls
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077333 bp, - strand  Genetic Position: Chr6, 52.73 cM
Col1a1tm1(tetO-Tcfap2c)Hsc/Col1a1+ Gt(ROSA)26Sortm1(rtTA*M2)Jae/Gt(ROSA)26Sor+ mice show increased proliferation and induced hepatic steatosis

Show the 4 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:98920
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Transactivator)
Mutation:    Insertion
Mutation detailsAn optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivatory protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920)
View phenotypes for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 12 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  291 strains or lines available
This mutation was originally created downstream of the Col1a1tm2(tetO-Pou5f1)Jae allele in mutant ES cell line KH2. The two mutations have subsequently been bred apart.

Original:  J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
All:  80 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.01
The Jackson Laboratory