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Targeted Allele Detail
Symbol: Gcktm3Mgn
Name: glucokinase; targeted mutation 3, Mark A Magnuson
MGI ID: MGI:3701764
Synonyms: GK(A456V)
Gene: Gck  Location: Chr11:5850820-5900081 bp, - strand  Genetic Position: Chr11, 3.88 cM
Germline Transmission:  Earliest citation of germline transmission: J:121910
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Not Applicable)
Mutation:    Nucleotide substitutions
Mutation detailsES cells were modified using a construct with an activating single base mutation introduced into exon 10 via site specific mutagenesis to change amino acid 456 from alanine to valine. (J:121910)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gck Mutation:  44 strains or lines available
On congenic B6 background, analysis is done on mice with allele with pgk-neo selection cassette deleted through mating to cre transgenic mice; on the coisogenic 129S6 background, allele retaining pgk-neo is analyzed, and authors state that presence of neo has no apparent effect on phenotype compared with mice on C57BL/6J congenic background. MMRRC has B6 congenic mice carrying Gcktm3Mgn allele (with neo deleted.)
Original:  J:121910 Pino MF, et al., Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. J Biol Chem. 2007 May 4;282(18):13906-16
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.19
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