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Targeted Allele Detail
Symbol: Gcktm2Mgn
Name: glucokinase; targeted mutation 2, Mark A Magnuson
MGI ID: MGI:3701697
Synonyms: GK(K414E)
Gene: Gck  Location: Chr11:5900820-5950081 bp, - strand  Genetic Position: Chr11, 3.88 cM
Germline Transmission:  Earliest citation of germline transmission: J:121910
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted
Mutation:    Nucleotide substitutions
Mutation detailsA construct with an inactivating single base pair mutation introduced into exon 9 via site specific mutagenesis to change amino acid 414 from lysine to glutamic acid was introduced into ES cells to generate mice. (J:121910)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gck Mutation:  26 strains or lines available
On congenic B6 background, analysis is done on mice with allele with pgk-neo selection cassette deleted through mating to cre transgenic mice; on the coisogenic 129S6 background, allele retaining pgk-neo is analyzed, and authors state that presence of neo has no apparent effect on phenotype compared with mice on C57BL/6J congenic background. MMRRC has 129S6 mice carrying Gcktm2Mgn (retaining neo cassette).

Original:  J:121910 Pino MF, et al., Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. J Biol Chem. 2007 May 4;282(18):13906-16
All:  1 reference(s)

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