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Transgene Detail
Symbol: Tg(CAG-Bgeo,-DsRed*MST)1Nagy
Name: transgene insertion 1, Andras Nagy
MGI ID: MGI:3697095
Synonyms: 11/C1, Tg(ACTB-Bgeo,-DsRed.MST)1Nagy, Tg(ACTB-Bgeo,-DsRed*MST)1Nagy, Tg(CAG-Bgeo,-DsRed*MST)1Nagy, Z/RED
Transgene: Tg(CAG-Bgeo,-DsRed*MST)1Nagy  Location: unknown  
Germline Transmission:  Earliest citation of germline transmission: J:109837
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
Mutation detailsThe transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed cre transgene results in fluorescence of ES cells, embryos and adult mice. (J:109837)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
The transgenic ES cell clone 11/C1 was used to generate transgenic mice.

The authors originally identified the red fluorescent protein variant employed in constructing the transgene vector as DsRed.T3; in a later erratum, they corrected this to DsRed-MST, which differs from the former at two amino acid positions (K Vintersten et al. 2005. Genesis 42(3):218).

see MMRRC Nagy ES cell lines: ES cell line G4 with DsRed.MST transgene (http://www.mmrrc.org/strains/11988/011988.html) and ES cell line G4 with Z/red transgene (http://www.mmrrc.org/strains/11987/011987.html) - dlb

Original:  J:109837 Vintersten K, et al., Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6
All:  21 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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