Tg(Cebpb-rtTA2S*S2)1Bjd
Transgene Detail

Summary

• Symbol: Tg(Cebpb-rtTA2S*S2)1Bjd
• Name: transgene insertion 1, Hermann Bujard
• MGI ID: MGI:3696443
• Synonyms: rTA^LAP, rTA^LAP-1, Tg(Cebpb-rtTA2S-S2)1Bjd
• Transgene: Tg(Cebpb-rtTA2S*S2)1Bjd Location: unknown
• Alliance: Tg(Cebpb-rtTA2S*S2)1Bjd page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F2

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene contains a cDNA with "humanized" codon usage translated from the amino acid sequence of a reverse tetracycline (tet) transactivator protein (rtTA2^S-S2) that was derived by the following modifications of the tet transactivator (repressor) protein (tTA): Glu19Gly, in the DNA binding domain; Ala56Pro, in the fourth alpha helix connecting the DNA binding domain with the core region; Asp148Glu and His179Arg, in the core near the tet-binding and dimerization domains, respectively. Expression is driven by the promoter of the gene encoding CCAAT/enhancer binding protein (C/EBP), beta, also called liver-enriched activating protein. The polyadenylation signal and a 3' intron are derived from a beta-globin gene. Progeny of mice doubly transgenic for this transgene and a tetracycline-operator- (tetO-) regulated luciferase gene exhibit no or barely detectable luciferase induction in the absence of doxycycline (Dox). Dox administration results in a rapid rise in luciferase activity, reaching 3 and 5 orders of magnitude in liver and kidney, respectively; histologic examination of livers of bitransgenic mice with a tetO-regulated lacZ gene revealed X-gal staining of all hepatocytes. Induction in other organs, including spleen, lung, heart, skeletal muscle and brain, is minimal. (J:81196, J:99884)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:81196 Schonig K, et al., Stringent doxycycline dependent control of CRE recombinase in vivo. Nucleic Acids Res. 2002 Dec 1;30(23):e134
• All: 14 reference(s)