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Transgene Detail
Symbol: Tg(APPSwLon)96Btla
Name: transgene insertion 96, Bruce Lamb
MGI ID: MGI:3693359
Synonyms: APPK670N/M671L and APPV717Im, J1.96, K670N/M671L + V717I, Tg(APP*Swe*Lon)96Btla
Transgene: Tg(APPSwLon)96Btla  Location: unknown  
Germline Transmission:  Earliest citation of germline transmission: J:42613
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(APPSwLon)96Btla expresses 1 gene
Mutation detailsA 650 kb YAC transgene containing the entire human amyloid beta (A4) precursor protein gene and approximately 250 kb of flanking sequence was altered to include the Familial Alzheimer's Disease- (FAD-) associated "Swedish" double mutation, APPK670N/M671L, and the "London" mutation, APPV717I. RT-PCR analysis shows hemizygous mice express the mutant human and endogenous mouse APP mRNA at similar levels in brain and peripheral tissues; levels of the most common alternative splice variants (encoding human APP-695, -751 and -770) parallel those of the endogenous mouse transcripts. Levels of total amyloid-beta and of longer amyloid-beta peptides (species terminating at amino acids 42/43) are higher than in wildtype mice, but not as high as in a similar strain carrying only the APPK670N/M671L "Swedish" mutant transgene. (J:42613)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
The transgene construct was injected into embryonic stem (ES) cells.
Original:  J:42613 Lamb BT, et al., Altered metabolism of familial Alzheimer's disease-linked amyloid precursor protein variants in yeast artificial chromosome transgenic mice. Hum Mol Genet. 1997 Sep;6(9):1535-41
All:  2 reference(s)

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MGI 6.12
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