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Slc6a3tm1(cre)Xz
Targeted Allele Detail
Nomenclature
Symbol: Slc6a3tm1(cre)Xz
Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1, Xiaoxi Zhuang
MGI ID: MGI:3691571
Synonyms: DAT-cre, Slc6a3Cre
Gene: Slc6a3  Location: Chr13:73536747-73578672 bp, + strand  Genetic Position: Chr13, 40.1 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:115426
Parent Cell Line:  W9.5/W95 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsA targeting vector was constructed by inserting a cassette with the cre recombinase coding sequence, a nuclear translocation signal, and a PGK-neo gene flanked by FRT sites, into the 5'-UTR of the Slc6a3 gene. After homologous recombination in ES cells, mice with cre recombinase expressed from the Slc6a3 gene were produced. Knockin mice were crossed with transgenic Flp deleter mice to remove the neo marker. A line was established where the neo was removed and the Flp transgene was absent. Cre recombinase expression is detected in the ventral tegmental area (VAT) and substantia nigra zona compacta (SNc). (J:115426)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Slc6a3     Summary of all recombinase alleles driven by Slc6a3.
 

Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 4 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Slc6a3 Mutation:  15 strains or lines available
References
Original:  J:115426 Zhuang X, et al., Targeted gene expression in dopamine and serotonin neurons of the mouse brain. J Neurosci Methods. 2005 Apr 15;143(1):27-32
All:  37 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/25/2015
MGI 6.0
The Jackson Laboratory