Tg(ACTB-GP33/NP396/bGal497/EGFP)69Gkl
Transgene Detail

Summary

• Symbol: Tg(ACTB-GP33/NP396/bGal497/EGFP)69Gkl
• Name: transgene insertion 69, George Kollias
• MGI ID: MGI:3690926
• Synonyms: ST33.396, Tg(ST33.396)69Gkl
• Transgene: Tg(ACTB-GP33/NP396/bGal497/EGFP)69Gkl Location: unknown
• Alliance: Tg(ACTB-GP33/NP396/bGal497/EGFP)69Gkl page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene produces a fusion protein consisting of three cytotoxic T lymphocyte (CTL) epitopes and enhanced green fluorescent protein under control of the ubiquitously expressing chicken beta-actin promoter. The CTL epitopes are: 1) amino acids 1-80 of the lymphocytic choriomeningitis virus (LCMV) glycoprotein, which include the immunodominant H2-D^b-restricted CTL epitope GP33-41; the H2-D^b-restricted LCMV nucleoprotein-derived epitope NP396-404; and the H2-K^b-restricted E. coli beta-galactosidase-derived epitope betaGal497-504. Cre recombinase-mediated removal of a loxP-flanked STOP cassette between the promoter and the coding sequence activates the gene. Although CD11c⁺ dendritic cells bearing this transgene and expressing Cre recombinase under control of the Itgax (Cd11c) promoter do not fluoresce, immunohistochemical analysis demonstrates that approximately 8% of them present the LCMV nucleoprotein epitope. (J:114742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:114742 Probst HC, et al., Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8+ T cell tolerance. Immunity. 2003 May;18(5):713-20
• All: 6 reference(s)