Tg(B19-RNAi:Il3)241Ckn
Transgene Detail

Summary

• Symbol: Tg(B19-RNAi:Il3)241Ckn
• Name: transgene insertion 241, Debra A Cockayne
• MGI ID: MGI:3689387
• Synonyms: AS-IL-3
• Transgene: Tg(B19-RNAi:Il3)241Ckn Location: unknown
• Alliance: Tg(B19-RNAi:Il3)241Ckn page

Transgene origin

• Strain of Origin: FVB/N X (BALB/c x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(B19-RNAi:Il3)241Ckn involves 1 genes/genome features (Il3) View all
• Mutation details: This transgene consists of the full length, 990-bp mouse interleukin 3 cDNA, in reverse (antisense) orientation, under control of the B19 parvovirus promoter and a T-cell dominant control region from the 3' end of the human CD2 gene. A human beta-globin gene segment including the 3' end of exon 2, all of intron 2 and exon 3, the polyadenylation signal and some 3' flanking DNA is present just downstream of the Il3 sequence. Interleukin 3 antisense RNA is detected by RT-PCR analysis in mature CD3⁺ T lymphocytes of transgenic animals both with lymphoproliferation and with no disease phenotype, in transgenic B220⁺IgM^- pre-B cells, and in brains of both asymptomatic and neurologically affected transgenic mice. Il3 antisense transcripts are present in cultured splenocytes from asymptomatic transgenic mice following in vitro concanavalin A (ConA) stimulation, and ELISA of supernatants from these cultures demonstrates a 5-fold reduction in interleukin 3 secretion compared to similar cultures of control splenocytes. (J:107618)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of 5 transgenic founder lines, two exhibited no abnormal phenotype and were not further characterized. One of the other founder mice died of neurologic disease at 3 months, without having bred. Founders 241 and 244 transmitted the transgene and both the lymphoproliferative and neurologic phenotypes to their progeny. The pathology of the neurologic and of the lymphoproliferative disorders differs between the two lines only in penetrance and in age of onset (3-6 months for line 241 compared to 6-12 months for line 244).

References

• Original: J:107618 Cockayne DA, et al., Transgenic mice expressing antisense interleukin-3 RNA develop a B-cell lymphoproliferative syndrome or neurologic dysfunction. Blood. 1994 Oct 15;84(8):2699-710
• All: 1 reference(s)