Tg(Tcra2B4)1Mmd
Transgene Detail

Summary

• Symbol: Tg(Tcra2B4)1Mmd
• Name: transgene insertion 1, Mark M Davis
• MGI ID: MGI:3688270
• Synonyms: 2B4alpha Tg, 2B4alphEH, Valpha11
• Transgene: Tg(Tcra2B4)1Mmd Location: unknown
• Alliance: Tg(Tcra2B4)1Mmd page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tcra2B4)1Mmd expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)

• Mutation details: A 2.3 kb fragment of the Valpha gene, containing 2 kb of 5' flanking sequence was fused to a 195 bp cDNA fragment covering the unique V-J junction, with the remaining nucleotides of the Jalpha region plus ~100 bp of JC intron sequence. This VJ segment was inserted upstream of a 9.8 kb Calpha clone containing all the Calpha exons and extending .5 kb downstream of the polyA signal. A 683-bp fragment containing the immunoglobulin heavy-chain enhancer (EH) was inserted at the junction between the VJ and Calpha segments. (J:69570)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:69570 Berg LJ, et al., Expression of T-cell receptor alpha-chain genes in transgenic mice. Mol Cell Biol. 1988 Dec;8(12):5459-69
• All: 13 reference(s)