Mkrn1^{Gt(RRB087)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mkrn1^{Gt(RRB087)Byg}
• Name: makorin, ring finger protein, 1; gene trap mutation RRB087, BayGenomics
• MGI ID: MGI:3665258
• Gene: Mkrn1 Location: Chr6:39374738-39397396 bp, - strand Genetic Position: Chr6, 18.18 cM
• Alliance: Mkrn1^{Gt(RRB087)Byg} page

Mutation origin

• Mutant Cell Line: RRB087
• Germline Transmission: Earliest citation of germline transmission: J:111785
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector inserted into intron 3. Gene-trapped transcripts contain a beta-geo fusion sequence, and lack exons 4-8. The encoded proteins lack four of the Makorin family zinc-finger motifs, including the essential RING E3 ubiquitin ligase domain. Histochemical visualization of the expressed fusion protein refined existing data that this gene is widely expressed and very abundant in specific cell types of the central nervous system and testis. (J:111785)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRB087	CC183243 ; (MGI Seq Detail)	5' RACE	Chr6:39382664-39397078 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  311 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mkrn1 Mutation: 122 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:111785 Gray TA, et al., The putatively functional Mkrn1-p1 pseudogene is neither expressed nor imprinted, nor does it regulate its source gene in trans. Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12039-44
• All: 5 reference(s)