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Tg(PF4-tTA/VP16)42Kra
Transgene Detail
Nomenclature
Symbol: Tg(PF4-tTA/VP16)42Kra
Name: transgene insertion 42, Katya Ravid
MGI ID: MGI:3656262
Synonyms: PF4-tTA-VP16
Transgene: Tg(PF4-tTA/VP16)42Kra  Location: unknown  
Transgene
origin
Strain of Origin:  FVB/NJ
Transgene
description
Transgene Type:    Transgenic (Transactivator)
Mutation:    Insertion
 
Mutation detailsThe rat platelet factor 4 (RP4) promoter was cloned into the tTA-containing plasmid, replacing the existing CMV promoter. The promoter will drive expression of the transactivator tTA fused to the viral coactivator VP16. Expression is repressed with administration of doxycycline and is induced by removal of doxycycline from the diet of the mice.The transgene also contains 1.7 kb of the 3'end of the human beta-globin gene including the 3' intron and polyadenylation signal to permit stable RNA production in mice. A stop codon in the transgene prevents translation of beta-globin into the protein. (J:104239)
Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:104239 Nguyen HG, et al., Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood. 2005 Sep 1;106(5):1559-64
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory