Oprd1^tm2Kff
Targeted Allele Detail

Summary

• Symbol: Oprd1^tm2Kff
• Name: opioid receptor, delta 1; targeted mutation 2, Brigitte L Kieffer
• MGI ID: MGI:3653589
• Synonyms: DOReGFP, DORGFP
• Gene: Oprd1 Location: Chr4:131838037-131871797 bp, - strand Genetic Position: Chr4, 64.78 cM, cytoband D1-D3
• Alliance: Oprd1^tm2Kff page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111033
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Homologous recombination was used to introduce the EGFP cDNA into exon 3 in frame and upstream of the stop codon. Western blot demonstrated expression of an EGFP protein as expected. Quantitative mRNA analysis demonstrated that the insertion did not interrupt normal transcription of the locus. (J:111033)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Oprd1 Mutation: 29 strains or lines available

References

• Original: J:111033 Scherrer G, et al., Knockin mice expressing fluorescent delta-opioid receptors uncover G protein-coupled receptor dynamics in vivo. Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9691-6
• All: 14 reference(s)