Rr165^tm2Crey
Targeted Allele Detail

Summary

• Symbol: Rr165^tm2Crey
• Name: regulatory region 165; targeted mutation 2, Claude-Agnes Reynaud
• MGI ID: MGI:3653480
• Synonyms: Igk^tm2Crey
• Gene: Rr165 Location: Chr6:70695543-70699506 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr165^tm2Crey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110775
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to mutate the KI-KII element (Rr163) such that 8 and 9 bp were replaced, respectively. The sequence of KI was mutated from TCCACGCATGCTTGGA to TGTACGCTGCTGATGA and KII was mutated from TCCTCTTCAGTGAGGAG to AGCTCTTCTAGACTGA. LoxP sites were inserted to surround 4 kb encompassing the promoter of the long J-C K germline transcript, located immediately upstream of the KI-KII element. (J:110775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr165 Mutation: 0 strains or lines available

References

• Original: J:110775 Cocea L, et al., A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain rearrangement in cis in mice and in the 103/bcl2 cell line. J Exp Med. 1999 May 3;189(9):1443-50
• All: 1 reference(s)