Sox1^tm1Asmi
Targeted Allele Detail

Summary

• Symbol: Sox1^tm1Asmi
• Name: SRY (sex determining region Y)-box 1; targeted mutation 1, Austin Smith
• MGI ID: MGI:3653406
• Synonyms: Sox1^GFP, Sox1-GFP knock-in, Sox1^KO
• Gene: Sox1 Location: Chr8:12445519-12449555 bp, + strand Genetic Position: Chr8, 5.73 cM, cytoband A1-A2
• Alliance: Sox1^tm1Asmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155046
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The open reading frame was replaced with EGFP, IRES, puromycin resistance cassette, and a floxed CMV-hygro cassette. Transient cre expression removed the hygro cassette. Expression of GFP in mice bearing this allele recapitulates the highly restricted pattern of Sox1 gene expression, which occurs initially in the neural plate and thereafter is maintained in neuroepithelial cells throughout the neuroaxis until it is downregulated during neuronal and glial differentiation. GFP expression from this allele similarly mimics that of Sox1 in vitro, being undetectable in undifferentiated ES cells but becoming apparent in a significant proportion of cells upon induction of neuronal differentiation by aggregation and retinoic acid treatment. (J:85845, J:155046)

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox1 Mutation: 13 strains or lines available

Notes

The ES cell line bearing this allele, named 46C, has been used by other investigators for further targeting. (J:155046) (J:85845)

References

• Original: J:85845 Aubert J, et al., Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1:11836-41
• All: 10 reference(s)