Bnc2^{Gt(pU21)18Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Bnc2^{Gt(pU21)18Imeg}
• Name: basonuclin zinc finger protein 2; gene trap 18, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3639337
• Synonyms: bnc2^-, GtAyu21-18, Gt(Ayu21)18Imeg
• Gene: Bnc2 Location: Chr4:84193332-84593512 bp, - strand Genetic Position: Chr4, 39.76 cM
• Alliance: Bnc2^{Gt(pU21)18Imeg} page

Small head, cleft palate and tongue abnormalities in Bnc2^{Gt(pU21)18Imeg}/Bnc2^{Gt(pU21)18Imeg} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: Ayu21-18
• Germline Transmission: Earliest citation of germline transmission: J:109358
• Parent Cell Line: KTPU10 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pU-21
• Mutation details: The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. The gene trap vector inserted into the intron between exon 2a and exon3. The absence of transcript expression was confirmed by RT-PCR. LacZ staining confirmed expression in the embryonic head and around the palatal cleft. (J:109358, J:151992)
• Inheritance: Not Specified
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  Ayu21-18	AB187235 ; (MGI Seq Detail)	5' RACE	Chr4:84593211-84593309 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  69 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	84 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Bnc2 Mutation: 130 strains or lines available

Notes

Integration site GenBank ID: AB187235

References

• Original: J:109358 Database for Exchangeable Gene Trap Clones, Information obtained from the Database for Exchangeable Gene Trap Clones (EGTC), an official database of the Gene Trap Project of the Institute of Molecular Embryology and Genetics and the Institute of Resource Development and Analysis, Kumamoto University, Japan. Unpublished. 2006
• All: 7 reference(s)