Hsf1^{Gt(pU18)54Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Hsf1^{Gt(pU18)54Imeg}
• Name: heat shock factor 1; gene trap 54, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3639095
• Synonyms: GtAyu18-54, Gt(Ayu18)54Imeg
• Gene: Hsf1 Location: Chr15:76361562-76385355 bp, + strand Genetic Position: Chr15, 35.95 cM
• Alliance: Hsf1^{Gt(pU18)54Imeg} page

Mutation origin

• Mutant Cell Line: Ayu18-54
• Germline Transmission: Earliest citation of germline transmission: J:109358
• Parent Cell Line: KTPU10 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped
• Mutation: Insertion of gene trap vector Vector: pU-18
• Mutation details: The randomly integrating (within a gene) gene trap vector pU18 contains 1.8 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by an internal ribosome entry site (IRES) and the Betageo beta-galactosidase/neomycin resistance fusion gene with a mammalian polyadenylation signal. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. Mutant and wild-type loxP sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. (J:109358, J:110088)
• Inheritance: Not Specified
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  Ayu18-54	AB212666 ; (MGI Seq Detail)	5' RACE	Chr15:76361627-76361917 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsf1 Mutation: 25 strains or lines available

Notes

Integration site GenBank ID: AB212666

References

• Original: J:109358 Database for Exchangeable Gene Trap Clones, Information obtained from the Database for Exchangeable Gene Trap Clones (EGTC), an official database of the Gene Trap Project of the Institute of Molecular Embryology and Genetics and the Institute of Resource Development and Analysis, Kumamoto University, Japan. Unpublished. 2006
• All: 3 reference(s)