Gt(pU21)105Imeg
Gene trapped Allele Detail

Summary

• Symbol: Gt(pU21)105Imeg
• Name: gene trap 105, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3639089
• Synonyms: GtAyu21-105, Gt(Ayu21)105Imeg
• Gene: Gt(pU21)105Imeg Location: Chr7:24539872-24539942 bp, - strand Genetic Position: Chr7, Syntenic
• Alliance: Gt(pU21)105Imeg page

Mutation origin

• Mutant Cell Line: Ayu21-105
• Germline Transmission: Earliest citation of germline transmission: J:109358
• Parent Cell Line: KTPU10 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped
• Mutation: Insertion of gene trap vector
• Mutation details: The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. (J:109358)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(pU21)105Imeg Mutation: 0 strains or lines available

Notes

Integration site GenBank ID: AB187256

References

• Original: J:109358 Database for Exchangeable Gene Trap Clones, Information obtained from the Database for Exchangeable Gene Trap Clones (EGTC), an official database of the Gene Trap Project of the Institute of Molecular Embryology and Genetics and the Institute of Resource Development and Analysis, Kumamoto University, Japan. Unpublished. 2006
• All: 1 reference(s)