2810004N23Rik^{Gt(pU21)55Imeg}
Gene trapped Allele Detail

Summary

• Symbol: 2810004N23Rik^{Gt(pU21)55Imeg}
• Name: RIKEN cDNA 2810004N23 gene; gene trap 55, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3630290
• Synonyms: GtAyu21-55, Gt(Ayu21)55Imeg
• Gene: 2810004N23Rik Location: Chr8:125566094-125589768 bp, - strand Genetic Position: Chr8, 72.81 cM, cytoband E2
• Alliance: 2810004N23Rik^{Gt(pU21)55Imeg} page

Mutation origin

• Mutant Cell Line: Ayu21-55
• Germline Transmission: Earliest citation of germline transmission: J:109358
• Parent Cell Line: KTPU10 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped
• Mutation: Insertion of gene trap vector Vector: pU-21
• Mutation details: The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. (J:109358)
• Inheritance: Not Specified
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  Ayu21-55	AB187244 ; (MGI Seq Detail)	5' RACE	Chr8:125587684-125588010 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  50 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 2810004N23Rik Mutation: 34 strains or lines available

Notes

Integration site GenBank ID: AB187244

References

• Original: J:109358 Database for Exchangeable Gene Trap Clones, Information obtained from the Database for Exchangeable Gene Trap Clones (EGTC), an official database of the Gene Trap Project of the Institute of Molecular Embryology and Genetics and the Institute of Resource Development and Analysis, Kumamoto University, Japan. Unpublished. 2006
• All: 3 reference(s)