Il2^tm1Hua
Targeted Allele Detail

Summary

• Symbol: Il2^tm1Hua
• Name: interleukin 2; targeted mutation 1, Hua Gu
• MGI ID: MGI:3630259
• Synonyms: Il-2-GFP^ki
• Gene: Il2 Location: Chr3:37174862-37180103 bp, - strand Genetic Position: Chr3, 18.3 cM, cytoband B-C
• Alliance: Il2^tm1Hua page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49411
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A green fluorescent protein (GFP) cDNA was inserted into the first exon such that the endogenous promoter directs expression of GFP. An adjacent selectable marker was removed by cre-mediated recombination in ES cells prior to the production of chimeric mice. Expression of GFP was shown to mimic endogenous Il2 gene expression in both a temporal and spatial manner; induction experiments also showed that GFP expression was triggered by costimulatory signals similar to the endogenous gene. (J:49411)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il2 Mutation: 41 strains or lines available

References

• Original: J:49411 Naramura M, et al., Mice with a fluorescent marker for interleukin 2 gene activation. Immunity. 1998 Aug;9(2):209-16
• All: 7 reference(s)