Akr1b7^tm1.1Fth
Targeted Allele Detail

Summary

• Symbol: Akr1b7^tm1.1Fth
• Name: aldo-keto reductase family 1, member B7; targeted mutation 1.1, Franz Theuring
• MGI ID: MGI:3629297
• Gene: Akr1b7 Location: Chr6:34389297-34400072 bp, + strand Genetic Position: Chr6, 14.95 cM
• Alliance: Akr1b7^tm1.1Fth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:123295
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A lox P site was inserted 5' of exon 2 and a floxed PGK-neomycin selection cassette was inserted 3' of exon 4. Transient cre expression in these ES cells resulted in excision of the Neomycin selection cassette as well as exons 2, 3, and 4. Northern blot analysis indicated the presence of a truncated mRNA. However, no protein product was detectable by Western blot analysis. (J:123295)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Akr1b7 Mutation: 24 strains or lines available

References

• Original: J:123295 Baumann C, et al., AKR1B7 (mouse vas deferens protein) is dispensable for mouse development and reproductive success. Reproduction. 2007 Jul;134(1):97-109
• All: 2 reference(s)