Runx1^tm1Medv
Targeted Allele Detail

Summary

• Symbol: Runx1^tm1Medv
• Name: runt related transcription factor 1; targeted mutation 1, Alexander Medvinsky
• MGI ID: MGI:3625885
• Synonyms: Runx1^LacZ
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm1Medv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106769
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A loxP flanked stop cassette was inserted downstream of the proximal promoter into the 5'UTR. To stop transcripts from the distal promoter, the cassette included a 1.7 kb splice acceptor (SA) sequence from the mouse Engrailed-2 gene. The NLS was placed in-frame between the upstream distal promoter exons and the Betagal gene, and transcription stop elements were included downstream of lacZ to prevent read-through of the cassette. The neo was excised by transient expression of cre recombinase. (The Engrailed2-SA-lacZ inserted sequence is flanked by loxP sites.) (J:106769)

Expression

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:106769 Samokhvalov IM, et al., Multifunctional reversible knockout/reporter system enabling fully functional reconstitution of the AML1/Runx1 locus and rescue of hematopoiesis. Genesis. 2006 Mar;44(3):115-21
• All: 8 reference(s)