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Cadm1tm1.2Brd
Targeted Allele Detail
Nomenclature
Symbol: Cadm1tm1.2Brd
Name: cell adhesion molecule 1; targeted mutation 1.2, Allan Bradley
MGI ID: MGI:3625158
Synonyms: Tslc1Brdm2, Tslc1m2
Gene: Cadm1  Location: Chr9:47530173-47857637 bp, + strand  Genetic Position: Chr9, 25.83 cM, cytoband B-C
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:108386
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsES cells targeted with a vector designed to flank exon 9 with loxP sites and insert an FRT-flanked neo were transfected with Cre recombinase. The floxed region including exon 9 and the neo were removed. Exon 9 encodes the transmembrane domain. The skipping of exon 9 resulted in a reading frame shift, which was predicted to result in a nonsense transcript. RT-PCR confirmed that the mutant allele was null. Immunohistochemistry demonstrated the absence of protein in mutant testis. (J:108386)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cadm1 Mutation:  23 strains or lines available
References
Original:  J:108386 van der Weyden L, et al., Loss of TSLC1 causes male infertility due to a defect at the spermatid stage of spermatogenesis. Mol Cell Biol. 2006 May;26(9):3595-609
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory