Tg(tetO-Gdnf,lacZ)14Brke
Transgene Detail

Summary

• Symbol: Tg(tetO-Gdnf,lacZ)14Brke
• Name: transgene insertion 14, Robert E Burke
• MGI ID: MGI:3623289
• Synonyms: BiTetO-LacZ-rGDNF
• Transgene: Tg(tetO-Gdnf,lacZ)14Brke Location: unknown
• Alliance: Tg(tetO-Gdnf,lacZ)14Brke page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: This transgene contains nucleotides 39-685 of the rat glial cell line derived neurotrophic factor (Gdnf) cDNA sequence (GenBank accession L15305) and the bacterial lacZ gene whose expression is driven by a tetracycline-responsive bidirectional promoter; each cDNA lies downstream of one of a pair of minimal promoters flanking a series of 7 copies of the tet operator (tetO) sequence. In doubly transgenic mice expressing the tetracycline transactivator or reverse transactivator protein in tissues of interest, expression of beta-galactosidase and GDNF can be regulated by administration of a tetracycline analog. Culture with striatal cells from transgenic mice induces exuberant sprouting in mesencephalic dopaminergic neurons, demonstrating functional activity of transgene-derived GDNF. (J:90075)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

X-gal staining of brains of doubly transgenic mice expressing the tetracycline transactivator gene (tTA) under control of the Camk2a promoter demonstrated expression of beta-galactosidase specifically in the striatum, cortex and hippocampus, where CAMK2 normally is expressed. This expression is suppressed by doxycycline administration. (J:90075)

Of two lines (#1 and #14) that expressed and transmitted the transgene, the phenotype in mice also bearing a transgene for a tetracycline transactivator has been characterized only for line 14.

References

• Original: J:90075 Kholodilov N, et al., Regulation of the development of mesencephalic dopaminergic systems by the selective expression of glial cell line-derived neurotrophic factor in their targets. J Neurosci. 2004 Mar 24;24(12):3136-46
• All: 1 reference(s)