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Tg(H2-D-Il15)3304Clgr
Transgene Detail
Nomenclature
Symbol: Tg(H2-D-Il15)3304Clgr
Name: transgene insertion 3304, Michael A Caligiuri
MGI ID: MGI:3623278
Synonyms: Dd-IL-15, Il15-Tg, IL15tg, IL-15tg
Transgene: Tg(H2-D-Il15)3304Clgr  Location: unknown  
Progressive alopecia in Tg(H2-D-Il15)3304Clgr/? mice

Show the 1 phenotype image(s) involving this allele.

Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(H2-D-Il15)3304Clgr expresses 1 gene
 
Mutation detailsThe coding region of the transgene comprises a cDNA encoding the mature mouse IL15 protein (nt 610-951) joined to the signal peptide-encoding sequence of the mouse IL2 gene (nt 49-108) and followed by a FLAG epitope tag coding sequence. Upstream of, and in frame with, the coding sequence is the nearly ubiquitously expressed promoter of the H2-Dd major histocompatibility class I gene, and downstream of and out of frame with it is the 3' end of the human growth hormone gene. RT-PCR and immunoblot analysis for the FLAG epitope detected high levels of mRNA and protein, respectively, in numerous tissues. Whereas IL15 is undetectable in sera of wild-type mice, measurable levels are present in ~75% of transgenic sera. (J:67478)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 11 anatomical structures
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Posttranscriptional checkpoints that maintain low endogenous wild-type IL15 intra- and extracellular protein levels - a series of 5' AUGs, the poorly translated and secreted IL15 signal peptide sequence and a C-terminal retention signal - have been eliminated from the transgene and replaced with sequences that maximize IL15 expression. (J:67478)

Although three transgenic lines were created in the FVB/N background, the phenotype is reported only for line 3304; the others are 3284 and 3285. It is stated that the phenotypes of all three are similar, but differ in severity depending on the level of transgene mRNA expression. (J:67478)

References
Original:  J:67478 Fehniger TA, et al., Fatal leukemia in interleukin 15 transgenic mice follows early expansions in natural killer and memory phenotype CD8+ T cells. J Exp Med. 2001 Jan 15;193(2):219-31
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/13/2021
MGI 6.16
The Jackson Laboratory