Rr320^tm5Mkg
Targeted Allele Detail

Summary

• Symbol: Rr320^tm5Mkg
• Name: regulatory region 320; targeted mutation 5, Mark Groudine
• MGI ID: MGI:3621031
• Synonyms: delta4neo, Hbb-ar^tm5Mkg
• Gene: Rr320 Location: Chr7:103501973-103526752 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: Rr320^tm5Mkg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106655
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to remove 5'HS4 encoded by nucleotides -19849 to -22561 relative to the Ey cap site. The removed sequence includes the core binding site, the region to which the HS maps and all regions of homology with human 5'HS1, however, three small blocks of homology of 7, 11, and 7 bp, upstream of the HS core remained after targeting. A loxP site flanked neomycin resistance gene cassette was included in the vector. The presence of the marker had a substantial effect on expression of beta-like globin genes at all stages. (J:106655)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr320 Mutation: 0 strains or lines available

References

• Original: J:106655 Bender MA, et al., Targeted deletion of 5'HS1 and 5'HS4 of the beta-globin locus control region reveals additive activity of the DNaseI hypersensitive sites. Blood. 2001 Oct 1;98(7):2022-7
• All: 1 reference(s)