Tg(MMTV-S100a4)463Oku
Transgene Detail

Summary

• Symbol: Tg(MMTV-S100a4)463Oku
• Name: transgene insertion 463, Olle Karlstrom
• MGI ID: MGI:3620787
• Synonyms: MMTVmts1, Tg463, Tg(MMTV-S100a4)463, TgN(Mmts1)463Oku
• Transgene: Tg(MMTV-S100a4)463Oku Location: unknown
• Alliance: Tg(MMTV-S100a4)463Oku page

Transgene origin

• Strain of Origin: CBA x C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(MMTV-S100a4)463Oku expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	S100a4 (MGI:1330282)

• Mutation details: A 2200 bp mouse genomic DNA fragment containing the S100 calcium binding protein A4 (S100a4) gene sequence, including the second intron and followed by an SV40 polyadenylation signal, was placed under transcriptional control of the long terminal repeat (LTR) of mouse mammary tumor virus (MMTV). Line 463 was shown by Southern blot analysis to have integrated 20-22 copies at a single site. Northern blot analysis demonstrates high levels of transgene-derived mRNA in lactating mammary glands of transgenic mice; the transcript also is present in salivary gland, thymus, kidney and lung and at unexpectedly high levels in spleen. S100A4 protein is clearly present by immunoblot analysis in lactating mammary glands of transgenic mice, but not of their wild-type littermates. (J:36642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:36642 Ambartsumian NS, et al., Metastasis of mammary carcinomas in GRS/A hybrid mice transgenic for the mts1 gene. Oncogene. 1996 Oct 17;13(8):1621-30
• All: 1 reference(s)