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Targeted Allele Detail
Symbol: Plxnb2tm1Matl
Name: plexin B2; targeted mutation 1, Marc Tessier-Lavigne
MGI ID: MGI:3620317
Synonyms: Plexin-B2-, Plxnb2-
Gene: Plxnb2  Location: Chr15:89155549-89180788 bp, - strand  Genetic Position: Chr15, 44.68 cM, cytoband E3
Perturbed olfactory bulb layering and proliferation in the Plxnb2tm1Matl/Plxnb2tm1Matl mice

Show the 3 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:119485
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
Mutation detailsTargeted mutagenesis using a promoterless gene trap vector ("targeted trapping") was used to insert a 9.4-kb placental alkaline phosphatase (PLAP) secretory cassette into the intron between exons 16 and 17 (counting the exon containing the translational start site as exon 1). This results in transcription of a fusion mRNA joining exons 1-16 of the endogenous gene to the elements of the cassette, comprising a splice acceptor and a transmembrane domain coding sequence followed by the betageo (lacZ/neomycin resistance fusion) cDNA, an internal ribosomal entry site (IRES), and the human PLAP sequence with a polyadenylation signal. Two proteins are translated from this bicistronic transcript: a PLXNB2/Betageo fusion protein that is sequestered in an intracellular compartment of the neuronal cell body; and PLAP, which is expressed on the axonal surface. (J:119485)
Inheritance:    Recessive
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 21 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Plxnb2 Mutation:  60 strains or lines available
On a primarily C57BL/6 background, homozygosity for this mutation causes perinatal death due to severe cranial neural tube closure defects that result in exencephaly, sometimes accompanied by a curled tail and/or spina bifida. Survivors among mutant mice of a mixed, partly (outbred) CD-1 background behave normally and even breed, although their cerebellar architecture is severly disrupted.
Original:  J:119485 Friedel RH, et al., Plexin-B2 controls the development of cerebellar granule cells. J Neurosci. 2007 Apr 4;27(14):3921-32
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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