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Transgene Detail
Symbol: Tg(Cp-EGFP)25Gaia
Name: transgene insertion 25, Nicholas R Gaiano
MGI ID: MGI:3620129
Synonyms: TNR, transgenic Notch reporter
Transgene: Tg(Cp-EGFP)25Gaia  Location: unknown  
Strain of Origin:  (C57BL/6J x SJL/J)F2
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
Mutation detailsThe transgene comprises four tandem copies of the C-promoter binding factor 1 (CBF1/RBPSUH) binding site of the Epstein-Barr virus major latency C promoter (Cp), followed by the SV40 minimal promoter and the coding sequence for enhanced green fluorescent protein. Cells of mice bearing this transgene express green fluorescent protein when the Notch signaling pathway is activated. (J:96367)
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
EGFP is expressed from this transgene when the promoter is activated by binding to the upstream CBF1 binding elements of a complex comprising CBF1, the transcriptional coactivator mastermind like 1 (Drosophila)(MML1), and the intracellular cleavage product resulting from interaction of the transmembrane Notch receptor with ligand.
Original:  J:96367 Duncan AW, et al., Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol. 2005 Mar;6(3):314-22
All:  42 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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