Tg(LIPC*S145G)1Ssf
Transgene Detail

Summary

• Symbol: Tg(LIPC*S145G)1Ssf
• Name: transgene insertion 1, Silvia Santamarina-Fojo
• MGI ID: MGI:3620047
• Synonyms: HL-S145G
• Transgene: Tg(LIPC*S145G)1Ssf Location: unknown
• Alliance: Tg(LIPC*S145G)1Ssf page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(LIPC*S145G)1Ssf expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LIPC (3990)

  Lipc	(MGI:96216)

• Mutation details: The transgene comprises a cDNA encoding the enzymatically nonfunctional S145G mutant human hepatic lipase (uniprot E7EUK6; NM_000236.3:c.616A>G;p.Ser206Gly) cloned between the promoter and polyadenylation signal of the human apolipoprotein E (APOE) gene; it also includes the hepatic and macrophage control regions from the Apoe/Apoc1 locus. Northern blot analysis demonstrated expression of the transgenic mRNA in liver, but not in any of 8 other organs/tissues examined. Western blot analysis revealed presence of the human protein in post-heparin plasma. The level of both mRNA and protein expression is 3- to 5-fold that of endogenous gene expression in wild-type mice. (J:94495)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Mutant human hepatic lipase lacking catalytic function but capable of binding ligand is expressed specifically in the liver.

References

• Original: J:94495 Gonzalez-Navarro H, et al., The ligand-binding function of hepatic lipase modulates the development of atherosclerosis in transgenic mice. J Biol Chem. 2004 Oct 29;279(44):45312-21
• All: 2 reference(s)