Tg(ACTB-ALPP)02Jrst
Transgene Detail

Summary

• Symbol: Tg(ACTB-ALPP)02Jrst
• Name: transgene insertion 02, James R Stringer
• MGI ID: MGI:3619344
• Synonyms: G11, G11 PLAP, Tg(ACTB-PLAP)02Jrst
• Transgene: Tg(ACTB-ALPP)02Jrst Location: unknown
• Alliance: Tg(ACTB-ALPP)02Jrst page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises a mutant human genomic placental alkaline phosphatase gene joined to the enhancer/promoter from the human beta-actin gene. The mutation was generated by inserting 7 G:C base pairs downstream of a series of 4 G:C pairs near the start of the reading frame, causing a frameshift that prevents production of a functional enzyme. Loss of a single base pair from the 11-G:C tract restores the reading frame and results in reversion to the wild-type enzymatic phenotype. (J:86017)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Histological analysis for heat-stable alkaline phosphatase activity identifies cells in which a deletional mutation has caused reversion of the mutant phenotype.

References

• Original: J:86017 DePrimo SE, et al., Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ. Methods. 1998 Sep;16(1):49-61
• All: 2 reference(s)