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Tcrbtm1Habu
Targeted Allele Detail
Nomenclature
Symbol: Tcrbtm1Habu
Name: T cell receptor beta chain; targeted mutation 1, Sonoko Habu
MGI ID: MGI:3618092
Synonyms: betaLD, Tcrbtm1Haub
Gene: Tcrb  Location: unknown  Genetic Position: Chr6, cytoband A-C
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:84291
Parent Cell Line:  TT2 (ES Cell)
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsTwo-step homologous recombination was used to delete 70 of the Tcrb locus (475 kb). The first targeting event replaced Dbeta1-Jbeta1 cluster with a loxP flanked neo and subsequent cre expression in the ES cell removed neo leaving a single loxP site. The second targeting event inserted another loxP flanked neo gene into the Vbeta1 exon. Subsequent cre expression in the selected ES clone removed large 475 kb region including the 3' half of the 5'Vbeta region, a central large intron of the locus and the first Dbeta-Jbeta cluster. (J:84291)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tcrb Mutation:  77 strains or lines available
References
Original:  J:84291 Senoo M, et al., Increase of TCR Vbeta accessibility within Ebeta regulatory region influences its recombination frequency but not allelic exclusion. J Immunol. 2003 Jul 15;171(2):829-35
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/12/2016
MGI 6.04
The Jackson Laboratory